| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.071229 |
| Chromosome: | chromosome 9 |
| Location: | 2992479 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g387550 | CGL142 | Conserved in the Green Lineage | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCCCCAGTTGTACAGAGCCGAGTGGGAGG |
| Internal bar code: | GGGGGTACAAAATGGGCTCCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 906 |
| LEAP-Seq percent confirming: | 99.2606 |
| LEAP-Seq n confirming: | 5101 |
| LEAP-Seq n nonconfirming: | 38 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATCAGGCTGAGCTGTGGTA |
| Suggested primer 2: | TCATCCTCCACGGACTTTTC |