| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.071261 |
| Chromosome: | chromosome 9 |
| Location: | 4509981 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g395880 | OPR11 | OctotricoPeptide Repeat protein 11 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCGTGACATATCAAAGAGGCGACTCCTGG |
| Internal bar code: | TGTTAGACAGCTGCTACTGCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4 |
| LEAP-Seq percent confirming: | 55.1471 |
| LEAP-Seq n confirming: | 150 |
| LEAP-Seq n nonconfirming: | 122 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGTAGCAAGAAGGGACAAG |
| Suggested primer 2: | TTCCTGTCTGTCACTGCGTC |