| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.071261 |
| Chromosome: | chromosome 14 |
| Location: | 811849 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g613100 | (1 of 1) IPR008999 - Actin cross-linking | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACAAGTGACACAGGCACTACAGTGGCCACT |
| Internal bar code: | TTCGCGGCACGACAAGCTGGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 547 |
| LEAP-Seq percent confirming: | 98.8024 |
| LEAP-Seq n confirming: | 1485 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGATGAGGAAAAAGGATGGA |
| Suggested primer 2: | ATGGTGTTGCTACCATGCAA |