| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.071273 |
| Chromosome: | chromosome 6 |
| Location: | 8223556 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g305700 | (1 of 14) IPR010998 - Integrase, Lambda-type, N-terminal | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGTGGCGATGCAGCCGGCAGCCGTACACG |
| Internal bar code: | CATGGAGCGAATGGAGTGTTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 374 |
| LEAP-Seq percent confirming: | 99.7358 |
| LEAP-Seq n confirming: | 1510 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCTCTCTTCGTGTCTGGTC |
| Suggested primer 2: | AAGCTAGCAAGGGTTAGGGC |