| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.071290 |
| Chromosome: | scaffold 18 |
| Location: | 199591 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre18.g749547 | (1 of 1) PF07510 - Protein of unknown function (DUF1524) (DUF1524) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCCGTGGGTGCAGGCGGTGCATTCTGTG |
| Internal bar code: | GGCGGGTGCGAGAGGGCGAAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 105 |
| LEAP-Seq percent confirming: | 74.4898 |
| LEAP-Seq n confirming: | 146 |
| LEAP-Seq n nonconfirming: | 50 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGAACAAAGACAGCGGTGA |
| Suggested primer 2: | TGCACAATGGTTCCAGTCAT |