| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.071332 |
| Chromosome: | chromosome 11 |
| Location: | 3409367 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g480725 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCTTGCTCGAAACCGAGACGTTGAACGA |
| Internal bar code: | AAGCGCCAAACACTTGCGCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 99 |
| LEAP-Seq percent confirming: | 61.5727 |
| LEAP-Seq n confirming: | 5403 |
| LEAP-Seq n nonconfirming: | 3372 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGGCTAGAGCAGCAATGGA |
| Suggested primer 2: | ACAGTAGCCCCAAAACATCG |