| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.071352 |
| Chromosome: | chromosome 9 |
| Location: | 2025366 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g394350 | NUP155 | Nucleoporin 155; (1 of 1) K14312 - nuclear pore complex protein Nup155 (NUP155, NUP170, NUP157) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTCTGCCGTGCGCCGCTTCCCCTCCCTCC |
| Internal bar code: | GAGTAGGGTTGAGTGTAGTAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 223 |
| LEAP-Seq percent confirming: | 99.0783 |
| LEAP-Seq n confirming: | 215 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCTACTGCGACCCTCTTG |
| Suggested primer 2: | GTAGATGAAGGCCGGTGTGT |