| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.071380 |
| Chromosome: | chromosome 15 |
| Location: | 1218586 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g641400 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAGGTCTCCAGGCCTGCGAGTGCCCCTTG |
| Internal bar code: | GCATCCCGTACGGGACGATTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 643 |
| LEAP-Seq percent confirming: | 96.9901 |
| LEAP-Seq n confirming: | 3029 |
| LEAP-Seq n nonconfirming: | 94 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTCGTTTCCAATCAACCAA |
| Suggested primer 2: | GGGCATGAAATTGCGTTACT |