| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.071385 |
| Chromosome: | chromosome 10 |
| Location: | 6286184 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g465000 | (1 of 8) 2.7.12.2 - Mitogen-activated protein kinase kinase / MKK | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGGTGAGCAGCGCTAGCGCTTGCGGTGAG |
| Internal bar code: | TATGGTGTGGGTCGGGTAGGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 211 |
| LEAP-Seq percent confirming: | 90.8815 |
| LEAP-Seq n confirming: | 897 |
| LEAP-Seq n nonconfirming: | 90 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTGGCTGGTTACGGTAGAA |
| Suggested primer 2: | AGTGTACACACGCGAGAACG |