Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.071405 |
Chromosome: | chromosome 12 |
Location: | 7723046 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre12.g553900 | CNG2 | (1 of 1) K04905 - potassium voltage-gated channel Eag-related subfamily H member 2 (KCNH2); Cyclic-nucleotide gated potassium channel | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGATGGGGCATTGTCACAAGCGCATCCGT |
Internal bar code: | TTGCATCTGTTGAGCACGTCTA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 962 |
LEAP-Seq percent confirming: | 99.3 |
LEAP-Seq n confirming: | 5958 |
LEAP-Seq n nonconfirming: | 42 |
LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CAGTCAAGATCACTTGCCGA |
Suggested primer 2: | ACCGACGACCACTATGGAAG |