| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.071419 |
| Chromosome: | chromosome 8 |
| Location: | 613691 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g359750 | RPS9 | Ribosomal protein S9, component of cytosolic 80S ribosome and 40 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGGCCTGCTCGTGTCCCCCAAGATGGGCC |
| Internal bar code: | GACACAGATCCCGGGGGAGTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 349 |
| LEAP-Seq percent confirming: | 99.5014 |
| LEAP-Seq n confirming: | 2794 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTCCTGTGAACCAGGTTTT |
| Suggested primer 2: | GTGCTAAGTGACGTTCGGGT |