| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.071425 |
| Chromosome: | chromosome 6 |
| Location: | 8127280 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g304913 | (1 of 14) PF01476 - LysM domain (LysM) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCGTTGGCGGAGCCGTTTGTGATGTTGGG |
| Internal bar code: | CCCACCATTTTTACGGCAAGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 900 |
| LEAP-Seq percent confirming: | 98.4872 |
| LEAP-Seq n confirming: | 1888 |
| LEAP-Seq n nonconfirming: | 29 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTAAATAAGCGGGGCCAAAT |
| Suggested primer 2: | CGTCATTACTGCCGTTGCTA |