| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.071426 |
| Chromosome: | chromosome 12 |
| Location: | 7015947 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g559750 | PRL2 | (1 of 14) PTHR10334 - CYSTEINE-RICH SECRETORY PROTEIN-RELATED; Predicted extracellular protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTTCCCTTCCGCTGACGCTTTCTTTCCCG |
| Internal bar code: | CGACGCCACCTGGATTGCAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 628 |
| LEAP-Seq percent confirming: | 99.9305 |
| LEAP-Seq n confirming: | 1437 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCCGAATGCACACAATCAC |
| Suggested primer 2: | ACTGTAACACGGATCCAGCC |