| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.071448 |
| Chromosome: | chromosome 14 |
| Location: | 2326345 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g623650 | ADH7 | Alcohol dehydrogenase; (1 of 3) 1.1.1.2 - Alcohol dehydrogenase (NADP(+)) / Aldehyde reductase (NADPH) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGATGTCGAACTTGCTCTTGTAGGCCGCGT |
| Internal bar code: | TTACGGGTGCCTAGGCGGCACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 185 |
| LEAP-Seq percent confirming: | 70.3963 |
| LEAP-Seq n confirming: | 302 |
| LEAP-Seq n nonconfirming: | 127 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGTCTGGGTCTCGGTTTAT |
| Suggested primer 2: | ATACGTGCGTGTGTGATCGT |