| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.071459 |
| Chromosome: | chromosome 6 |
| Location: | 8223870 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g305700 | (1 of 14) IPR010998 - Integrase, Lambda-type, N-terminal | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACCGACGTACCGCAAATGCCATCGCGCAT |
| Internal bar code: | CCAGGGGCAACCGGCCCCACGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 605 |
| LEAP-Seq percent confirming: | 99.7588 |
| LEAP-Seq n confirming: | 7446 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGCTAGCAAGGGTTAGGGC |
| Suggested primer 2: | GGCTCTCTTCGTGTCTGGTC |