| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.071466 |
| Chromosome: | chromosome 5 |
| Location: | 2118599 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g234640 | (1 of 1) IPR000048//IPR011989//IPR016024 - IQ motif, EF-hand binding site // Armadillo-like helical // Armadillo-type fold | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGATGTCGCGAGCCGCCGCCTCCTCACGCA |
| Internal bar code: | CCTGCAAAGGCGACCGTGCTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 129 |
| LEAP-Seq percent confirming: | 84.8739 |
| LEAP-Seq n confirming: | 101 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGTCCGAACCTCTTCTTG |
| Suggested primer 2: | CACTTCCTCTACCCTCACGC |