| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.071470 |
| Chromosome: | chromosome 12 |
| Location: | 8827627 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g545101 | XDH1 | (1 of 1) 1.17.1.4 - Xanthine dehydrogenase / Xanthine/NAD(+) oxidoreductase; Xanthine dehydrogenase/oxidase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACGTCCCCGTCTTGCTAACCCATGTCTCT |
| Internal bar code: | GGCGGGATAAGCGATACTCAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5 |
| LEAP-Seq percent confirming: | 94.7777 |
| LEAP-Seq n confirming: | 1833 |
| LEAP-Seq n nonconfirming: | 101 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGTGTGTGTGTGTGTGTGT |
| Suggested primer 2: | TGAACTCGTCATTCTCGTGC |