| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.071496 |
| Chromosome: | chromosome 10 |
| Location: | 1455754 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g428650 | CDPKK1,CDPKKK1 | (1 of 3) K07359 - calcium/calmodulin-dependent protein kinase kinase (CAMKK); Calcium/calmodulin-dependent protein kinase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTCTCCCTGGCGCATGATGATGTCGCCCT |
| Internal bar code: | CGCGGGAGGGGTCTTTACCGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 234 |
| LEAP-Seq percent confirming: | 75.8347 |
| LEAP-Seq n confirming: | 1908 |
| LEAP-Seq n nonconfirming: | 608 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCGACACTACGGTAAAGGT |
| Suggested primer 2: | ACCCTACGCAATAATGCAGC |