| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.071502 |
| Chromosome: | chromosome 1 |
| Location: | 4423386 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g030000 | (1 of 1) PTHR12242:SF1 - PROTEIN ROLLING STONE | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCAGCCACGCCTACTGCTACGCCGCCCCA |
| Internal bar code: | GCTGTGTTGGGCCCTTTCGGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 218 |
| LEAP-Seq percent confirming: | 99.5035 |
| LEAP-Seq n confirming: | 2004 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACAGAAGGCACCATCCTCC |
| Suggested primer 2: | CCAGCAGAGGAGGTATGCTC |