Insertion junction: LMJ.RY0402.071522_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.071522
Chromosome:chromosome 11
Location:1245046
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre11.g467702 MRPS3,uS3m (1 of 3) PF00189 - Ribosomal protein S3, C-terminal domain (Ribosomal_S3_C); Mitochondrial ribosomal protein S3 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCGCGCGCATCCGCCGCAACCAGCCCCGA
Internal bar code:AATCATCCGATCGATGCAGCCC

Confirmation - LEAP-Seq

LEAP-Seq distance:104
LEAP-Seq percent confirming:86.2857
LEAP-Seq n confirming:151
LEAP-Seq n nonconfirming:24
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:AGTCATAACGGCACCTGACC
Suggested primer 2:GTGACAAACACGAGAGCGAA