| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.071555 |
| Chromosome: | chromosome 5 |
| Location: | 1416750 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g244200 | TMA1,COTH2 | spore coat protein H; (1 of 3) PF08757 - CotH protein (CotH) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACACAAACCGCACCTGCAGCCGCGCCTGC |
| Internal bar code: | GTAGATCGTTTATGATGGCGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 642 |
| LEAP-Seq percent confirming: | 99.6986 |
| LEAP-Seq n confirming: | 2977 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAGCTGTTGTATGGGGAGG |
| Suggested primer 2: | ACGACTTTACAAAGCCCCCT |