| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.071558 |
| Chromosome: | chromosome 10 |
| Location: | 4511940 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g452050 | LHCA4 | (1 of 1) K08908 - light-harvesting complex I chlorophyll a/b binding protein 2 (LHCA2); light-harvesting protein of photosystem I | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGACAGAAGGCGAAACAATAGAAACCACA |
| Internal bar code: | GATCGTGGTCATAGCTTTTGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 579 |
| LEAP-Seq percent confirming: | 99.1329 |
| LEAP-Seq n confirming: | 343 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTTCTTGCTAAGTCCTCCG |
| Suggested primer 2: | CTGCCTCCCATATGCAAACT |