| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.071570 |
| Chromosome: | chromosome 12 |
| Location: | 3240150 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g498600 | EEF1,EEF1A1 | (1 of 3) K03231 - elongation factor 1-alpha (EEF1A); Eukaryotic translation elongation factor 1 alpha | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGCGGTGGTCCTACACGTCGGCGGTAGCT |
| Internal bar code: | GATCCAGGTTGACGTAAAGTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1228 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 457 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGACTCCTTCAAGAACTGCG |
| Suggested primer 2: | GCTGTCGTACAGCATCCTCA |