| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.071570 |
| Chromosome: | chromosome 12 |
| Location: | 6052658 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g535350 | (1 of 1) IPR011044//IPR017986 - Quinoprotein amine dehydrogenase, beta chain-like // WD40-repeat-containing domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGTAAGCAGGGCGGGGCGCTGGGACGTGG |
| Internal bar code: | TTACCGGCGGATTTGACGGTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 133 |
| LEAP-Seq percent confirming: | 21.4123 |
| LEAP-Seq n confirming: | 94 |
| LEAP-Seq n nonconfirming: | 345 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAGACCCCTTGACACCCTA |
| Suggested primer 2: | AATCAGGTCATCCAACAGCC |