Insertion junction: LMJ.RY0402.071585_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.071585
Chromosome:chromosome 16
Location:221074
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre16.g694703 CGL57 (1 of 1) PTHR12383//PTHR12383:SF5 - PROTEASE FAMILY S26 MITOCHONDRIAL INNER MEMBRANE PROTEASE-RELATED // PEPTIDASE-S24/S26 DOMAIN-CONTAINING PROTEIN; Serine protease of S26A family CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCAGCGCCCGCCTCCTCCCCGGCTCTCCCG
Internal bar code:CTGAACGGCACTTCGCACCGCA

Confirmation - LEAP-Seq

LEAP-Seq distance:43
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:46
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:TGCAAGTTCTTTGCCATCTG
Suggested primer 2:GCTGCAAGGATGTTTGGAAT