| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.071607 |
| Chromosome: | chromosome 13 |
| Location: | 5116085 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g607450 | DAK1 | Dihydroxyacetone kinase; (1 of 1) 2.7.1.28 - Triokinase / Triose kinase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGAGGCGCGCCGCGGGCGGCTGCGGCGCCG |
| Internal bar code: | TCCGCCGGACGCTGGGGGGTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 136 |
| LEAP-Seq percent confirming: | 58.7302 |
| LEAP-Seq n confirming: | 37 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACAGGCTTCACAGTGACCA |
| Suggested primer 2: | CGCTCCTTCTATCCCCTACC |