| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.071609 |
| Chromosome: | chromosome 2 |
| Location: | 5942443 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g111900 | (1 of 20) IPR008972 - Cupredoxin | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCTTTCGTCAAGGGCGGCAGATGAGGAGA |
| Internal bar code: | CCTGCGCCGATAGAACTCGCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 327 |
| LEAP-Seq percent confirming: | 99.7553 |
| LEAP-Seq n confirming: | 2446 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTAAAGTTGAAGCAGGGCG |
| Suggested primer 2: | GAACTGCACATGAGGCAAGA |