| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.071637 |
| Chromosome: | chromosome 3 |
| Location: | 8898134 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g207825 | (1 of 1) K03241 - translation initiation factor eIF-2B subunit gamma (EIF2B3) | 3'UTR|outside_mRNA | |
| Cre03.g207937 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGCGGGTCGTGTCAGTCCTGACTGTCAC |
| Internal bar code: | CAGATGCAAGGTGAAACGGTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 335 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 141 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACATTACGTCCGCATGACA |
| Suggested primer 2: | GTCTTGACCAGGTATGCCGT |