| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.071652 |
| Chromosome: | chromosome 13 |
| Location: | 1588482 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g573300 | CDD4 | (1 of 1) K11991 - tRNA(adenine34) deaminase [EC:3.5.4.33] (tadA); Cytosine deaminase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGACGGCGGCCAGCCGCAATGGTTGGGTGT |
| Internal bar code: | CCAAAACATTGACTTAGCAAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 567 |
| LEAP-Seq percent confirming: | 99.6372 |
| LEAP-Seq n confirming: | 1648 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCCTCCTCCACAGTAGCAA |
| Suggested primer 2: | AGAGGCCGCTTGAATAGTGA |