| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.071664 |
| Chromosome: | chromosome 1 |
| Location: | 6209447 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g044350 | (1 of 2) PTHR11319//PTHR11319:SF28 - G PROTEIN-COUPLED RECEPTOR-RELATED // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTGGGACCCATGTGGTGTCGCATGATGCA |
| Internal bar code: | ACGTAAGATTGAAATGGGGACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 607 |
| LEAP-Seq percent confirming: | 95.695 |
| LEAP-Seq n confirming: | 1556 |
| LEAP-Seq n nonconfirming: | 70 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCGTCGTGCTACTGTTGGA |
| Suggested primer 2: | CACCTCGATGAAGCTGTTGA |