Insertion junction: LMJ.RY0402.071688_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.071688
Chromosome:chromosome 14
Location:2658638
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre14.g626400 CYP33,CYP743C1 (1 of 3) 1.14.14.1//5.3.99.5 - Unspecific monooxygenase / Xenobiotic monooxygenase // Thromboxane-A synthase / Thromboxane synthetase; Cytochrome P450, CYP197 superfamily intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCAGGGGTGAGGGAGGAGGGGTGCGGGGGT
Internal bar code:CTGATGCTCGTCGCGACGGCTG

Confirmation - LEAP-Seq

LEAP-Seq distance:29
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:8
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CGATCTACGTGGCTGTCTCA
Suggested primer 2:AATCCGTGTTGCGTTCCTAC