Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.071690 |
Chromosome: | chromosome 9 |
Location: | 7057075 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre09.g411450 | DAP1,DNAAF2,Ktu,MOT45,PF13,PRR1 | (1 of 3) PF08190 - pre-RNA processing PIH1/Nop17 (PIH1); Dynein Assembly factor PIH domain 1 | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGCCCGCCCCAAGCTGCCACACCCCCACC |
Internal bar code: | TCGTTGCACGAGGAAAGGGCGG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 82 |
LEAP-Seq percent confirming: | 97.3684 |
LEAP-Seq n confirming: | 37 |
LEAP-Seq n nonconfirming: | 1 |
LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GTGTCTGTGGGTGCGTTATG |
Suggested primer 2: | ACACGTACACGCACACAGGT |