| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.071727 |
| Chromosome: | chromosome 16 |
| Location: | 2479145 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g660750 | MKS6,CC2D2A | (1 of 1) K19352 - coiled-coil and C2 domain-containing protein 2A (CC2D2A); Coiled-coil and C2 domain containing 2A | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGGTGGCGCTGCTGGGGCGGGCGCCGCGG |
| Internal bar code: | CCGACGATTCAGAGTGGTGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 28 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 49 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTACCATGACCACTCCCTGG |
| Suggested primer 2: | AGCTCAAGGAGGAGGAGGAG |