| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.071742 |
| Chromosome: | chromosome 10 |
| Location: | 1096690 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g425501 | (1 of 1) K03125 - transcription initiation factor TFIID subunit 1 (TAF1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TATGGGAGGAGAGGGCGGGGACCAGCGGGG |
| Internal bar code: | GTGGGCTACAGCAATGGGATCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 199 |
| LEAP-Seq percent confirming: | 93.3954 |
| LEAP-Seq n confirming: | 2107 |
| LEAP-Seq n nonconfirming: | 149 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATTCGATGTCCCGCTAGAT |
| Suggested primer 2: | ATGTGAAGAGGTGGCTGGAC |