| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.071742 |
| Chromosome: | chromosome 13 |
| Location: | 4151923 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g591900 | PLD6,PLD1 | (1 of 1) K16862 - mitochondrial cardiolipin hydrolase [EC:3.1.4.-] (PLD6); putative mitochondrial cardiolipin hydrolase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGCCGGGGACAAAGGGCGAGCGGCGGGCT |
| Internal bar code: | ACGCTTCTCCATGGTGCTTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 596 |
| LEAP-Seq percent confirming: | 99.7677 |
| LEAP-Seq n confirming: | 859 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGATGATCCTCGTGCTGATT |
| Suggested primer 2: | GTAAGCCTGCATTGCTCCTC |