| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.071747 |
| Chromosome: | chromosome 7 |
| Location: | 5374897 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g350100 | (1 of 1) IPR000104//IPR006311//IPR017956//IPR026741//IPR026937//IPR027417 - Antifreeze protein, type I // Twin-arginine translocation pathway, signal sequence // AT hook, DNA-binding motif // Protein strawberry notch // Strawberry notch, helicase C domain // P-loop containing nucleoside triphosphate hydrolase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGCTGGTCTCGAAGCTGAACGAGTCGAAC |
| Internal bar code: | AGGAAGAGGCTCCGCGGACTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 501 |
| LEAP-Seq percent confirming: | 99.7789 |
| LEAP-Seq n confirming: | 1354 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCCCCTCTACACACACACA |
| Suggested primer 2: | GGTACTGTTGGGCTTGTGGT |