| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.071762 |
| Chromosome: | chromosome 16 |
| Location: | 2039271 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g657000 | (1 of 1) K03134 - transcription initiation factor TFIID subunit 10 (TAF10) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGCTAGTTGTATGTAAACAAATGGTTTGC |
| Internal bar code: | ACCGGAGTGACAACCGCAACTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 54 |
| LEAP-Seq percent confirming: | 64.4444 |
| LEAP-Seq n confirming: | 58 |
| LEAP-Seq n nonconfirming: | 32 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACACTCCAGTCATCAGGGC |
| Suggested primer 2: | CGCTAGCTGCTTACCAATCC |