Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.071765 |
Chromosome: | chromosome 7 |
Location: | 743842 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre07.g317800 | (1 of 1) PF04091 - Exocyst complex subunit Sec15-like (Sec15) | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGAAGTGCGAACCGAATCCCGCGCTGCCG |
Internal bar code: | CAACTCACCTCGGAGCTGCGAT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 876 |
LEAP-Seq percent confirming: | 99.4675 |
LEAP-Seq n confirming: | 4296 |
LEAP-Seq n nonconfirming: | 23 |
LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TTACCCAGCACGCCTAATTC |
Suggested primer 2: | GCAGGCCAAACGACTCTTAG |