| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.071770 |
| Chromosome: | chromosome_5 |
| Location: | 1380259 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre05.g244350 | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GGTTGTGCATTGTCTTGCCTTGCCCTACGT |
| Internal bar code: | CGTCGCGGTACGCGTAACTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 770 |
| LEAP-Seq percent confirming: | 85.4545 |
| LEAP-Seq n confirming: | 94 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGAACATACATGCAACCAC |
| Suggested primer 2: | AAGTGGTATGCCGGACAAAG |