| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.071772 |
| Chromosome: | chromosome 17 |
| Location: | 2060510 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g711750 | HTB5,HTB22 | Histone H2B | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCACCTGGTAACTCAGCCCTCATTAGCTTT |
| Internal bar code: | CATTCAACAAAAGGTCGAGGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 573 |
| LEAP-Seq percent confirming: | 99.4258 |
| LEAP-Seq n confirming: | 1039 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCAACACCACCCTCAACCT |
| Suggested primer 2: | CTAACACCTCCTGGCATGGT |