| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.071784 |
| Chromosome: | chromosome 17 |
| Location: | 2021820 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g711401 | (1 of 10) IPR006593 - Cytochrome b561/ferric reductase transmembrane | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTACATACCCATCTGTCAACGCCGGTGGC |
| Internal bar code: | GAATCATCTAGGCGACACAGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 228 |
| LEAP-Seq percent confirming: | 24.077 |
| LEAP-Seq n confirming: | 613 |
| LEAP-Seq n nonconfirming: | 1933 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCTTATAACCTGGGGCATT |
| Suggested primer 2: | CGGCAATGAGTCTCTCACAA |