| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.071822 |
| Chromosome: | chromosome_12 |
| Location: | 3372352 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre12.g497250 | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TCGTCTAGTCGATGGCGCAAGAAGCCCTCG |
| Internal bar code: | CTGGGGGCACTCGTTCGGAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 215 |
| LEAP-Seq percent confirming: | 90.6694 |
| LEAP-Seq n confirming: | 447 |
| LEAP-Seq n nonconfirming: | 46 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTACCAACACACTCGAGCA |
| Suggested primer 2: | TTGACGCCATACAAGCTGAG |