| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.071825 |
| Chromosome: | chromosome 10 |
| Location: | 4307426 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g450850 | (1 of 1) PF00168//PF16016 - C2 domain (C2) // Domain of unknown function (DUF4782) (DUF4782) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAACTAGCTATGCTTACACCTCAAAATATA |
| Internal bar code: | TATACAGGGCCCTCGGGGGGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 565 |
| LEAP-Seq percent confirming: | 99.3249 |
| LEAP-Seq n confirming: | 1177 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACTGTGCGGGATATGTGTG |
| Suggested primer 2: | CGCTTCTGGTAGGCAGGTAG |