| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.071855 |
| Chromosome: | chromosome 17 |
| Location: | 3574549 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g725600 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAGCGCCAGGAATCGAAACGGCTCGAACT |
| Internal bar code: | GTCAGGAAACCAAAACGACGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 525 |
| LEAP-Seq percent confirming: | 99.6964 |
| LEAP-Seq n confirming: | 3940 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTCGGTCTGGTTTGGTGAT |
| Suggested primer 2: | CTAACGACTTAAGCAGGCCG |