| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.071857 |
| Chromosome: | chromosome 10 |
| Location: | 809854 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g423300 | (1 of 1) 3.4.22.40 - Bleomycin hydrolase / Aminopeptidase C (Lactococcus lactis) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAGGGACTCCCGCCATTCCCGTGAACACG |
| Internal bar code: | ACACATACCTGCGTGCTGGCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 154 |
| LEAP-Seq percent confirming: | 96.3297 |
| LEAP-Seq n confirming: | 1601 |
| LEAP-Seq n nonconfirming: | 61 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGACCAGGATAAGGTGTGT |
| Suggested primer 2: | TAGTTGCAAGCGATAATGCG |