| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.071862 |
| Chromosome: | chromosome 10 |
| Location: | 4523445 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g452150 | (1 of 100) IPR001810 - F-box domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGCGCTCCTGTGGACACACCCCATACGGT |
| Internal bar code: | CAGGTCCGAGTAACATGGATCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 174 |
| LEAP-Seq percent confirming: | 99.6471 |
| LEAP-Seq n confirming: | 847 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGTACTTTCTGCCGTTCCC |
| Suggested primer 2: | ATCGGCAAAGAGCAGGACTA |