| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.071898 |
| Chromosome: | chromosome 10 |
| Location: | 356142 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g420450 | (1 of 2) PTHR18841:SF0 - PROTEIN C08G5.3, ISOFORM A-RELATED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGCCATGCACTGCCCGGAGAGCGAATGGC |
| Internal bar code: | GTTTCCGGGTGTGGAACAGGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 182 |
| LEAP-Seq percent confirming: | 94.9109 |
| LEAP-Seq n confirming: | 4420 |
| LEAP-Seq n nonconfirming: | 237 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAACCCACTACGCACACAC |
| Suggested primer 2: | GTGCTGCGATATGAAAGGGT |