| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.071898 |
| Chromosome: | chromosome 16 |
| Location: | 923934 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g648451 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGACGATGGGTTGTGAATACCAGCCCAAA |
| Internal bar code: | TATAGGATTGGTCCCGCGTCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 640 |
| LEAP-Seq percent confirming: | 70.3704 |
| LEAP-Seq n confirming: | 304 |
| LEAP-Seq n nonconfirming: | 128 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAGACACAAGCACACAGGC |
| Suggested primer 2: | ACGGGCAGTTTCAATCAGAC |