| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.071905 |
| Chromosome: | chromosome 10 |
| Location: | 2033388 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g432800 | RPSA,RPSa | Ribosomal protein Sa, component of cytosolic 80S ribosome and 40 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTGTGTCAAAACCTGGGAGGGGGCTGAAG |
| Internal bar code: | AAATAACGGATGATTTTCGATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 861 |
| LEAP-Seq percent confirming: | 99.1883 |
| LEAP-Seq n confirming: | 1222 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTGTCGCCCAGAAACAAAC |
| Suggested primer 2: | CGACAGGAGACATTTGCTCA |