| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.071913 |
| Chromosome: | chromosome 1 |
| Location: | 4422389 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g030000 | (1 of 1) PTHR12242:SF1 - PROTEIN ROLLING STONE | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCAGCTGAGAGGGGGAGCTTTGCGCTACA |
| Internal bar code: | CAAGTCCAATTTATTCGTTGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 796 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 488 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCCACAAATGTCTTCAGCC |
| Suggested primer 2: | TCTGTCGGATCAGCAGAATG |